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Usually, development of a new biotherapeutic entails developing a new method for analyzing various properties, such as glycosylation.  Glycans are instrumental in cellular signalling, a specific protein's glycosylation can influence the effectiveness of the biotherapeutic.  Given the importance of glycosylation to the overall performance of a glycoprotein, there is a need to completely characterize and then monitor these glycans for both research and manufacturing purposes. Many recombinant proteins, such as recombinant human erythropoietin (rhEPO), have highly intricate glycosylation patterns consisting of O-linked glycosylation as well as complex, branched N-linked glycosylation with three different N-linked glycosylation sites. The heterogeneity of N-linked glycosylation is mainly attributed to differences in sialylated glycans, which make approaches such as intact mass by high resolution mass spectrometry virtually impossible. This presents a challenge as the presence of sialic acid is critical in the characterization of a sialylated glycoprotein, as well as for the removal of excess labelling reagent, salts, or surfactants.

The Preciex® Glycan enrichment Stage-tip uses an anion exchange Glycocapture Resin to capture high-mannose, hybrid, and complex glycans from cleavage intact protein for effective downstream analysis by FLR, MALDI and ESI-MS. Traditional sample preparation involves intensive manipulation and sample loss, but with a Glycan stage-tip product, the results are sensitive and effective, saving time. The benefit of strong hydrophilic interaction is critical to bind 🔥fluorophore-labelled glycans, like highly polar sialylated glycans. 

Process of use: centrifugation, vacuum, and positive pressure.Formats: 10µL and 200µL.For any other format or online trap column, contact us.  *200µL tips are available for different Liquid Handling Automation systems (Zymark, Multiprobe, Tecan, Hamilton, Gilson, Qiagen, and many more) without any further adaptor needed.  

Features of Glycan Stage-tip:

• Higher selectivity for glycans
• Low volume elutions of up to 5 μL for directly shooting into MS.
ᩚᩚᩚᩚᩚᩚ⁤⁤⁤⁤ᩚ⁤⁤⁤⁤ᩚ⁤⁤⁤⁤ᩚ𒀱ᩚᩚᩚ• Work efficiently with sialylated glycoprotein

• Suitable for manual and automated processing

Common Product Specifications:

Sample	Glycans
Chromatographic bed	AX resin
Volume	200μL
Capacity	2ug
pH Range	2-8
Temperature	4-60°C
Quantity	96 Tips
Detection Method	🐠HPLC, Mass spectrometry, Capillary electrophoresis, Atomic absorption spectroscopy

Ordering Information:

Description	Format	Capacity	Part Number
Preciex Glycan	10μL	0.5μg	2110001
Preciex Glycan	200μL	2μg	2110022
Preciex Glycan	200μL	10μg	2110103

Specifically, we provide an approach that is fast and reliable to analyse Sialylated O-glycans, which is the most labile glycan class due to its sensitivity of pH, alkaline β-elimination, sample handling and mass spectrometry ionization. An in-depth understanding structure-function relationship of these glycoproteins will speed up learning about the immune system, developing therapeutic biological drugs and vaccines.

Ordering Information:

Description	Format	Capacity	Part Number
Preciex Sialylated Glycan	10μL	0.5μg	2111001
Preciex Sialylated Glycan	200μL	2μg	2111022
Preciex Sialylated Glycan	200μL	10μg	2111103

User Guide

Material Safety Data Sheets (MSDS)